Certified Drug Test Collector Training

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The Drug Test Collector plays a critical role in the workplace drug screening process. Along with the employer, the testing facility and the Medical Review Officer (MRO), the collector is an essential part of a system developed to ensure drug-free workplaces for the sake of public safety.

As the collector, you are the only individual in the drug-testing process who has direct, face-to-face contact with the employee. You ensure the integrity of the urine specimen and collection process and begin the chain of custody that includes the laboratory; the MRO; the employer; and, possibly, the courts.

This training is a professional-level course that provides the knowledge and skills to qualify Drug Test Collectors to perform U.S. Department of Transportation-regulated drug tests and non-regulated tests. Course participants also have the option of becoming professionally certified after completion of this course. This designation confirms that the collector is committed to the highest standards in the drug and alcohol testing industry.

The Course

This professional-level course meets the regulatory standards of U.S. Department of Transportation (DOT) rule 49 CFR Part 40 and provides a solid foundation for a wide range of testing programs. 

Course ComponentsDeliverablesApplicability
Library of terms & resourcesUniversal skills setMultiple industries
LessonsDOT QualificationPublic sector 
Short quizzes & final examinationProfessional CertificationPrivate sector 
Mock collections Regulated by local, state and federal authorities

How to Take the Course

The Drug Test Collector Training involves multiple parts that need to be completed in a specific order to achieve certification.

1. Before starting the training, the collector must:

2. Take the course Pre-Test to show familiarity with the subject matter based on a review of the materials provided. 

2. Complete the lessons of the training along with the required short quizzes.

3. Take the final exam. A score of at least 90 percent is required.

4. When you pass the online portion of this training, continue to the Next Steps lesson for instructions on how to set up five mock collections with a live examiner. These must be scheduled within 30 days of course completion and are required for qualification and certification.

5. Once the mock collections are completed without error, you will be qualified and can perform both federally regulated and non-regulated drug test collections.

6. To be certified, qualified collectors are asked to sign an agreement promising to adhere to the standards set in the training. The course administrator will then issue a certification form documenting that the collector is both a USDOT Qualified and Professionally Certified Drug Testing Collector. Contact the course administrator for more information.


Reference Library

This section provides materials for download that will support successful completion of the course.

Glossary of Terms

Terms and abbreviations the collector will commonly come across during the drug testing process.

Drug Test Collector Training Pre-Test

A pre-test is required at the start of the course to show that course participants have reviewed DOT Urine Specimen Collection Guidelines and 49 CFR Part 40, as is required by federal law.

What you Need to Know About 49 CFR Part 40

This lesson provides an overview of 49 CFR Part 40, which outlines rules and procedures for conducting workplace drug and alcohol testing for the federally regulated transportation industry.

Introducing the Custody and Control Form

The Custody and Control Form (CCF) is the essential tool for tracking and documenting each step in the drug testing process. This lesson is the first of six covering the parts of the form that the collector is responsible for.

Preparing the Collection Enclosure

One of the collector’s key responsibilities is to eliminate opportunities for the donor tamper with the specimen. In this course we outline strategies for securing the collection enclosure.

Starting the Collection

In this lesson we provide an overview of each step that is necessary to ensure the integrity of the specimen from the time the employee arrives for the test until they hand the specimen to the collector.

Handling the Specimen

This lesson provides an overview of the chain of custody from the moment the donor provides the specimen to the time the collector transfers the sample to the specimen bottles and seals and signs them.

Completing the Collection

This lesson covers the final steps of the collection process including transfer of custody of the specimen.

Atypical Collection: Adulterated Specimen Protocol

A key indication that a sample may have been tampered with during the collection process is when the sample is not within the expected temperature range. This lesson outlines what the collector needs to do in event of an out-of-range temperature reading.

Atypical Collection: Insufficient Specimen Protocol

To complete a drug test successfully, the employee must provide at least 45 ml of urine. This lesson provides an overview of what the collector must do if the donor does not provide the mandated quantity or is not able to provide a specimen.

Atypical Collection: Uncooperative Donor

There are times when an employee refuses to comply with the testing process or certain steps in the testing process. This lesson provides the collector with guidance on how to handle an uncooperative donor and what to do if the collection process can’t be completed.

Collection Flaws and How to Avoid Them

Errors in a drug test can lead to wasted time and money for employers, bad feelings for employees, distrust, and ultimately, a less safe workplace because of incomplete testing. This lesson covers fatal and correctable flaws and how to avoid them.

Collector Certification Next Steps

Length: 10 minutesComplexity: Easy

Congratulations on successfully completing the online portion of the Certified Drug Collector Training course and passing the exam. According to federal guidelines, within 30 days of completing the course, you must set up five mock collections with an approved trainer to become a qualified drug test collector. This can be done virtually or in person. The Process… View Article